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Product Details
Synonyms
FIGF, VEGFD
Source
Biotinylated Mouse VEGF-D Protein, His,Avitag (VED-M82E4) is expressed from human 293 cells (HEK293). It contains AA Phe 98 - Ser 206 (Accession # P97946).
Predicted N-terminus: Phe 98
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 19.6 kDa. The protein migrates as 28-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Mouse VEGF-D Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (Cat. No. FL4-M5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF-D Protein, His,Avitag (Cat. No. VED-M82E4) with a linear range of 10-78 ng/mL (QC tested).
Protocol
Immobilized Mouse VEGF R2 Protein, Mouse IgG2a Fc Tag (Cat. No. VE2-M5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF-D Protein, His,Avitag (Cat. No. VED-M82E4) with a linear range of 0.01-0.156 μg/mL (Routinely tested).
Protocol
Immobilized Mouse VEGF R1 Protein, Mouse IgG2a Fc Tag (Cat. No. VE1-M5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF-D Protein, His,Avitag (Cat. No. VED-M82E4) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Mouse VEGF R3 Protein, Mouse IgG2a Fc Tag (Cat. No. FL4-M5251) captured on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Mouse VEGF-D Protein, His,Avitag (Cat. No. VED-M82E4) with an affinity constant of 2.13 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Vascular endothelial growth factor D
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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