Save Product
Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteT7 RNA polymeraseFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity<0.78 ng/MlStandard Curve Range0.78 ng/mL-50 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants.Sample volume100 μLProduct Overview
resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is designed to detect and quantitatively determine T7 RNA Polymerase residues in mRNA drugs. The kit contains T7 RNA Polymerase (ACROBiosystems, cat# T7E-E5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling T7 RNA Polymerase residues during the production of mRNA products. It can also be used as a universal detection tool for the quantitative determination of T7 RNA Polymerase.
Storage
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES018-C01Pre-coated Anti-T7 RNA polymerase Antibody Microplate1 plate (8×12 strips)RES018-C02T7 RNA polymerase Standard100 μLRES018-C03Biotin-Anti-T7 RNA polymerase Antibody150 μLRES018-C04Streptavidin-HRP50 μLRES018-C0520xWashing Buffer50 mLRES018-C06Biotin-Antibody and Streptavidin-HRP Dilution Buffer50 mLRES018-C07Standard and Sample Dilution Buffer (5x)30 mLRES018-C08Substrate Solution12 mLRES018-C09Stop Solution7 mLACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Intra-Assay Statistics
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Recovery
Three T7 RNA polymerases with different concentrations were tested to calculate the recovery rate.
Customer Reviews Writing Reviews
Contact Us
Drug Candidate Licensing