His Product List
Host Species
- Mouse（10）
Conjugate
- FA Labeled（3）
- Acridinium ester Labeled（1）
- AF488 Labeled（1）
- Biotin-labeled（1）
- Europium chelate Labeled（1）
- HRP Labeled（1）
- PE Labeled（1）
- Unconjugated（1）
Reactive Species
- -（4）
- His tag (N‑terminal, C‑terminal, internal His‑tagged fusion proteins)（3）
- Tag（2）
- His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)（1）
Applications
- ELISA（3）
- Flow（2）
- MPCLIA（1）
- WB（1）
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FAQ
What should I consider if the His-tag signal is weak or undetectable?
A weak or undetectable signal may be related to factors such as His-tag accessibility, assay format, sample preparation, or detection conditions. Confirm that the selected antibody is suitable for your assay and His-tag configuration, and optimize experimental conditions as needed. For product-specific recommendations, refer to the corresponding product information.
Can ACROBiosystems' anti-His tag antibodies be used for SPR assays?
Yes. Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) is validated for use in SPR, in addition to ELISA and Western blot.
When should I choose a directly labeled or conjugated anti-His antibody?
Directly labeled or conjugated anti-His antibodies can simplify assay workflows by enabling direct detection without an additional labeled secondary antibody. ACROBiosystems offers multiple labeling options, including HRP, Alexa Fluor™ 488, PE, biotin, acridinium ester, FA, and Europium chelate, allowing you to select a format that matches your detection platform and assay design.
Which anti-His reagents are available for TR-FRET assays?
ACROBiosystems offers FA-labeled anti-His tag antibodies (Cat. Nos. FRT-TA04, FRT-TA05, and FRT-TA11) and a Europium chelate-labeled anti-His tag antibody (Cat. No. FRT-TD05) for TR-FRET assays. Select the appropriate reagent combination according to your TR-FRET assay design.
Which anti-His tag antibodies can be used for flow cytometry?
ACROBiosystems offers directly labeled anti-His antibodies for flow cytometry, including Alexa Fluor™ 488-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8) (Cat. No. HIS-AFM549) and PE-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8) (Cat. No. HIS-PFM549).
Which anti-His tag antibodies are suitable for ELISA and Western blot?
Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) is validated for ELISA and WB, as well as SPR. For direct enzyme-based detection, HRP-conjugated Monoclonal Anti-His Antibody, Human IgG1 (AY63) (Cat. No. HIS-PLM535) is available for ELISA and WB.
Can ACROBiosystems' anti-His antibodies recognize N-terminal, C-terminal, and internal His tags?
Yes. Selected ACROBiosystems anti-His antibodies recognize His tags located at the N-terminus, C-terminus, or internally within His-tagged fusion proteins. Please refer to the individual product specifications for validated reactivity.
How do I choose the right anti-His tag antibody for my application?
Choose an anti-His tag antibody based on your assay format and detection method. ACROBiosystems offers anti-His antibodies for ELISA, Western blot (WB), SPR, flow cytometry, TR-FRET, and MPCLIA, with a range of unlabeled and directly labeled formats to support different experimental needs.
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Clinical Drug Information
| English Name | Research Code | Highest Development Stage | Company | Indication | Clinical Trial |
|---|---|---|---|---|---|
| Praluzatamab ravtansine | CX-2009 | Phase 2 Clinical | Immunogen Inc, Cytomx Therapeutics Inc | Solid tumours; Ovarian Neoplasms; Head and Neck Neoplasms; Neoplasms; Triple Negative Breast Neoplasms; Breast Neoplasms; Carcinoma, Non-Small-Cell Lung | Details |
Customer Reviews
E***t2026-04-21We ordered and tested the anti-His antibody from ACROBiosystems for routine Western blot and ELISA experiments, and we were very satisfied with both the product quality and the delivery time.- R***n2026-02-21I have ordered, ACROBiosystemsHRP conjugated Anti-His Antibody (AY63), mAb, for my ELISA experiment, working very good! May I have a PINK color llama? Thanks, -Raven