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Product Details
Synonym
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (CDE-HA2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1-2.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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Background
Its primary function is to act as a crucial structural scaffold, mediating proper interactions with other CD3 subunits (specifically CD3G and CD3D).
It is considered as SP34 binding site.
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD3 epsilon chain
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
6 Details
- Highest Development Stage：
Approved
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