Productos etiquetados con fluorescencia Star Staining
Característica de producto
Puede cubrir los sitios activos para reducir la bioactividad
Fácil de agregar debido al cambio de hidrofilia de las proteínas
Alta variación en la consistencia de lote a lote
Mantener la conformación y modificación natural.
Tecnología eficientemente bio-ortogonal
Alta consistencia y uniformidad de lote a lote.
Características de Star Staining
ACROBiosystems se esfuerza por desarrollar reactivos de detección de alto estándar para apoyar el desarrollo de la terapia con células RAQ-T. Después de años de investigación y experiencia por parte de nuestros técnicos, establecimos con éxito la plataforma de etiquetado específica del sitio "Star Staining" y desarrollamos una serie de proteínas exclusivas marcadas con fluorescencia con calidad "Star Standard". Los productos de "Star Staining" pueden ser herramientas poderosas para detectar y monitorear las células RAQ-T en ensayos clínicos.
Uso de tecnología de etiquetado específica del sitio de nueva generación para mantener la bioactividad natural
Alta especificidad y sensibilidad verificadas por citometría de flujo
Sin unión no específica a PBMC no transducidas
Alta consistencia y uniformidad de lote a lote
Productos Más Productos
Visualización de datos
Alta pureza
FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.
Alta bioactividad
Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.
The activity of Alexa Fluor™ 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) was higher than other Competitor.
The activity of Alexa Fluor™ 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.
Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.
Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.
Unión no específica a PBMC no transducidas
Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
Mantener la bioactividad natural
Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.
Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.
Alta consistencia entre lotes
Binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H6) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
Alta estabilidad
PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) 25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.
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REFERENCIAS
[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print,2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.
[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.
[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.
- Introducción
- Característica de producto
- Características de Star Staining
- Productos Más Productos
- Visualización de datos