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ELISA Solutions: High-Performance Buffers and Nickel-Coated Plates

In ELISA assays, where precision and data reliability are critical, researchers often encounter challenges such as elevated background signals, suboptimal standard curve linearity, and complex assay workflows. These issues are frequently attributed to variations in buffer composition and the quality of key reagents. Even minor inconsistencies can substantially affect assay sensitivity, specificity, and reproducibility. To overcome these obstacles, we have developed a comprehensive range of ELISA buffers and nickel-coated plates, providing stable, high-performance solutions that ensure consistent results across both routine and specialized applications.

Buffers

Buffers
In ELISA assays, critical steps such as blocking, washing, and sample dilution depend on robust and reliable buffers. Our optimized ELISA buffer effectively blocks non-specific binding sites on microplates, substantially reducing background signals and enhancing the assay’s signal-to-noise ratio. Each component is rigorously validated for compatibility to ensure seamless performance across all assay steps, supporting a broad dynamic range and excellent reproducibility. Furthermore, our dilution buffer is specifically formulated to minimize non-specific adsorption—including serum-induced background—thereby maximizing signal clarity and detection sensitivity.
Product List
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EBS-001 ELISA Buffer Set-96T

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EBS-002 ELISA Buffer Set (Phosphate system, 96T)

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DB-02 Dilution Buffer (Strengthen Blocking)

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Application Data
Validated by ELISA, the Dilution Buffer effectively reduces serum-derived background signals in SARS-CoV-2 Spike RBD detection

Validated by ELISA, the Dilution Buffer effectively reduces serum-derived background signals in SARS-CoV-2 Spike RBD detection

2% serum diluted by Dilution Buffer (Cat.No. DB-02 ): Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 2.383 and 2.148; 2% serum diluted by DB-02: Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 0.262 and 0.263. In the existence of DB-02, the serum background values significantly decreased. Note: Dilution Buffer is Wash Buffer with 0.5% (w/v) bovine serum albumin (BSA), 50 mL.

Protocol

Nickel-Coated Plates

Nickel-Coated Plates
For studies and applications involving His-tagged recombinant proteins, our nickel-coated plates offer a highly efficient and convenient detection solution. The surface is densely coated with nickel ions, enabling specific and high-affinity capture of His-tagged proteins while providing excellent binding capacity and minimal non-specific adsorption. This design effectively reduces background interference and enhances data reliability. With the nickel-coated plates, researchers can perform capture and detect target proteins directly without conventional coating steps, greatly simplifying the experimental workflows. They are particularly well suited for rapid quantification, activity analysis, and interaction screening of recombinant proteins.
Product List
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SP-19 Nickel Coated Plates, Clear, 12×8-Well Strips, White Frame

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Application Data
Nickel-coated plates: suitable for evaluating the binding activity between Siglec-10 protein and anti-Siglec-10 antibody

Nickel-coated plates: suitable for evaluating the binding activity between Siglec-10 protein and anti-Siglec-10 antibody

Immobilized Cynomolgus Siglec-10, His Tag at 1 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips (Cat.No. SP-19), can bind Anti-Cynomolgus Siglec-10 Antibody, Human IgG1 with a linear range of 0.005-0.313 μg/mL, CV% of Intra-assay tests＜10% (QC tested).

Protocol
Nickel-coated plates: suitable for assessing the binding activity between GLP1R protein and anti-GLP1R antibody

Nickel-coated plates: suitable for assessing the binding activity between GLP1R protein and anti-GLP1R antibody

Immobilized Human GLP1R Full Length Protein (L260F), Flag,His Tag at 5 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips (Cat.No. SP-19), the binding activity with Monoclonal Anti-GLP1R Antibody, Fc Tag (A6727) was the similar with the other nickel plate.

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  • ELISA Solutions: High-Performance Buffers and Nickel-Coated Plates
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