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Immunohistochemistry (IHC) Antibody

Immunohistochemistry (IHC) is an antibody-based technique that utilizes the specific binding between antigens and antibodies. Through enzyme-labeled antibodies and chromogenic substrate reactions, it enables the localization, qualitative, and quantitative analysis of antigens within tissue cells. Due to its high specificity, sensitivity, and precise localization, IHC effectively integrates morphological and functional studies, making it widely applied across various fields of biological and medical research.

Due to its ability to visually and  qualitatively detect target proteins in tissue samples, IHC has become an essential validation method in the development of solid tumor-targeted therapies such as antibody-drug conjugates (ADCs) and TIL-based cell therapies. To better support drug development efforts, ACROBiosystems, through its ACRODiagnostic pathology platform, has conducted targeted IHC methodological validation for its antibody products, ensuring their specificity and suitability for IHC applications. Validation is performed using both healthy and tumor tissue samples to confirm accurate staining localization and applicability across representative cancer types. Furthermore, to meet diverse needs, our antibody development and pathology platforms offer customized IHC antibody development and screening services, allowing tailored solutions based on specific research requirements.

Product Features

Clinical-Focused Product Portfolio for Hot Biotherapeutics

Clinical-Focused Product Portfolio for Hot Biotherapeutics

Focus on antibody development targeting Top Antibody & ADC therapy core targets, aligning with biomarker detection needs in clinical trials.

Clinical-Oriented IHC Validation System

Clinical-Oriented IHC Validation System

Validate across multiple assay methods and diverse pathology sample types that strictly comply with practical IHC clinical requirements.

Stable IHC Antibody Performance

Stable IHC Antibody Performance

Ensure exceptional specificity, sensitivity, lot‑to‑lot uniformity, and accurate subcellular localization that are benchmarked against pathological diagnostic standards, supporting reliable IHC results.

Stable Supply Chain Assurance

Stable Supply Chain Assurance

Maintain stable supply chain for Stable raw materials, ensuring consistent production and supply of high-quality products.

Target Overview of IHC Antibody Products

Head & Neck Cancer (Thyroid Cancer, HNSCC)
Thoracic Cancer (NSCLC, SCLC, Breast Cancer)
Gastrointestinal Cancer (Colorectal, Gastric, Pancreatic)
Urological Cancer (Renal Cell, Urothelial Carcinoma)
Reproductive System Cancer (Prostate, Ovarian Cancer)

Product List

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Validation Data & Application Example

Claudin-18.2 IHC Antibody（Cat. No. HCS-S278）
Example of cross-reactivity: According to the Claudin-18.2 immunohistochemical cross-reactivity experiment, there is no cross-reactive staining result between Claudin-18.2 and Claudin-18.1.
> Use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissue, strong positive staining of gastric gland cell cytoplasm and/or cell membrane, negative staining of other tissue cells.

Human Stomach Tissue, 4X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10)(HCS-S278)Human Stomach Tissue, 4X

Human Stomach Tissue, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Stomach Tissue, 20X
> Normal lung tissue is stained with Claudin-18.2 primary antibody (Cat. No. HCS-S278), and lung tissue cells are negatively stained (Claudin-18.2 normal lung 20X).

Human Lung Tissue, 4X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Lung Tissue, 4X

Human Lung Tissue, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Lung Tissue, 20X

* The human gene Claudin-18 has two protein isoforms, Claudin-18.1 and Claudin-18.2, which differ within the N-terminal 69 amino acids. Claudin-18.1 is predominantly expressed in the lungs, whereas Claudin-18.2 is predominantly expressed in the stomach.

Staining example of human tumor tissue samples: use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain gastric cancer, colorectal cancer，pancreatic cancer, and ovarian cancer tissue samples respectively
> Using Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissues, positive cell cytoplasm and/or cell membrane specific positive staining (staining intensity ≥ 1), no non-specific staining (staining intensity < 1) , no background staining (staining intensity < 1 ).

Human Gastric Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Gastric Cancer, 20X

Human Colorectal Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10)(HCS-S278)Human Colorectal Cancer, 20X

Human Pancreatic Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Pancreatic Cancer, 20X

Human Ovarian Cancer, 20X

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) -Recombinant Monoclonal Anti-Claudin-18.2 Antibody, Mouse (3B10) (HCS-S278)Human Ovarian Cancer, 20X
Staining example of applicable platform: use Claudin-18.2 primary antibody (Cat. No. HCS-S278) to stain normal gastric tissues at Leica BOND-III and Dako link 48.

Leica BOND-III-10X

Leica BOND-III-10X

Leica BOND-III-40X

Leica BOND-III-40X

Dako link 48-10X

Dako link 48-10X

Dako link 48-40X

Dako link 48-40X
Performance comparison
Titer: ACRO at 1/1000 dilution and Company A at 1/200 dilution, color intensity is similar
> Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Claudin-18.2 with ACRO/Company A. Membranous staining on human gastric epithelial cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Claudin18.2 (ACRO)
1:1000-40X

Claudin18.2 (ACRO)1:1000-40X

Claudin18.2 (Company A)
1:200-40X

Claudin18.2 (Company A)1:200-40X

Claudin18.2 (Company A)
1:500-40X

Claudin18.2 (Company A)1:500-40X
Sensitivity: ACRO staining intensity is stronger than Company A
> Immunohistochemical analysis of paraffin-embedded human gastric cancer labeling Claudin-18.2 with ACRO/Company A. Membranous staining on tumor cells is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Claudin18.2 (ACRO)
1:1000-4X

Claudin18.2 (ACRO)1:1000-4X

Claudin18.2 (Company A)
1:200-4X

Claudin18.2 (Company A)1:200-4X

Claudin18.2 (Company A)
1:500-4X

Claudin18.2 (Company A)1:500-4X

Claudin18.2 (ACRO)
1:1000-40X

Claudin18.2 (ACRO)1:1000-40X

Claudin18.2 (Company A)
1:200-40X

Claudin18.2 (Company A)1:200-40X

Claudin18.2 (Company A)
1:500-40X

Claudin18.2 (Company A)1:500-40X

FAQ

Q

What are ACROBiosystems IHC antibodies used for?

They are used to detect and visualize specific target proteins in tissue samples, enabling the assessment of protein expression and localization. They support biomarker analysis and validation in cancer research, particularly in the development of targeted therapies such as antibody-drug conjugates (ADCs) and cell therapies.
Q

Which targets are covered by the IHC antibody portfolio?

Examples include CLDN18.2, HER2, EGFR, TROP-2, NECTIN-4, PSMA, FOLR1, and immune checkpoint markers.
Q

How should I select an IHC antibody clone?

Choosing one validated for your specific application, sample type (e.g., FFPE or frozen tissue), and species. Review published data, staining images, specificity, and recommended protocols to ensure reliable results. A clone validated for IHC in your target tissue is generally the best choice. If multiple clones are available, compare their validation data and literature rather than choosing based on the clone number alone.
Q

Are healthy and tumor tissues included in IHC validation?

Yes. The validation approach uses healthy and tumor tissue samples to assess staining localization and applicability.
Q

Which example addresses CLDN18.2 isoform specificity?

Clone 3B10, HCS-S278, has tissue staining examples addressing CLDN18.2 versus CLDN18.1 cross-reactivity.
Q

Are automated IHC staining examples available?

Yes. Automated IHC staining examples are available for selected antibodies, including Claudin-18.2 tested on the Leica BOND-III and Dako LINK 48 platforms.
Q

Can one dilution be used for all IHC antibodies?

No.The optimal dilution varies depending on the antibody, target expression, sample type, and staining conditions. Please refer to the recommended dilution in the product datasheet and optimize as needed.
Q

Can IHC antibodies be developed for additional targets?

Custom IHC antibody development and screening services are available for defined research requirements.
  • Background
  • Product Features
  • Target Overview of IHC Antibody Products
  • Product List
  • Validation Data & Application Example
  • FAQ