Solutions for Herpesviruses Vaccine Development
Multimeric Structural Antigen Designed based Native Conformation
Diverse Options: Various herpesvirus antigens are available, including gB, gC, gD, gE, gH & gL, gH & gL & gO, etc.
Natural Conformation: Eukaryotic HEK293 cells express proteins & natural conformation designed proteins.
High Homogeneity: The antigen proteins exhibit high homogeneity verified by SEC-MALS.
Glycoprotein H & Glycoprotein L/gH & gL (VZV)
The purity of Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-V5283) is more than 90% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.
Glycoprotein I & Glycoprotein E/gI & gE (VZV)
The purity of Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) was greater than 85% as determined by SEC-HPLC.
Glycoprotein C/gC (HSV)
The purity of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (Cat. No. GLC-V52H3) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Glycoprotein H & Glycoprotein L/gH & gL (HSV)
The purity of HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (Cat. No. GHL-H5283) is more than 85% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Glycoprotein H & Glycoprotein L/gH & gL (EBV)
The purity of Epstein-Barr virus Glycoprotein H&Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (Cat. No. GHL-E5283) is more than 90% and the molecular weight of this protein is around 100-125kDa verified by SEC-MALS.
Glycoprotein B/gB (EBV)
The purity of Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (Cat. No. GLB-E52H3) is more than 85% and the molecular weight of this protein is around 275-305 kDa verified by SEC-MALS.
Glycoprotein H & Glycoprotein L/gH & gL (HCMV)
The purity of HHV-5 (strain AD169) Glycoprotein H&L, His Tag (Cat. No. GHL-H5243) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Glycoprotein B/gB (HCMV)
The purity of HCMV (strain AD169) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H4) is more than 95% and the molecular weight of this protein is around 308-376 kDa verified by SEC-MALS.
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| Product Type | Virus | Molecule | Cat. No. | Product Description |
|---|---|---|---|---|
| Antigen | VZV | Glycoprotein H & Glycoprotein L/gH & gL (VZV) | GHL-V5283 | Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified) |
| Glycoprotein I & Glycoprotein E/gI & gE (VZV) | GIE-V5283 | Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (HPLC-verified) | ||
| Glycoprotein E/gE (VZV) | GLE-V52H3 | Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag (MALS verified) | ||
| HSV | Glycoprotein H & Glycoprotein L/gH & gL (HSV) | GHL-H5283 | HSV-1 (strain 17) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified) | |
| Glycoprotein B/gB (HSV) | GLB-H52H3 | HSV-2 (strain 333) Glycoprotein B (gB), His Tag (MALS verified) | ||
| Glycoprotein C/gC (HSV) | GLC-V52H3 | HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) | ||
| Glycoprotein D/gD (HSV) | GLD-V52H3 | HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (MALS verified) | ||
| Glycoprotein D/gD (HSV) | GLD-V52H4 | HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (MALS verified) | ||
| Glycoprotein E/gE (HSV) | GLE-H52H4 | HSV-2 (HG52) Glycoprotein E Protein, His Tag (MALS verified) | ||
| Glycoprotein B/gB (HSV) | POB-V52H3 | HSV-1 (strain KOS) post-gB Protein, His Tag (MALS verified) | ||
| EBV | Glycoprotein H & Glycoprotein L/gH & gL (EBV) | GHL-E5283 | Epstein-Barr virus Glycoprotein H&Glycoprotein L Heterodimer Protein, Twin-Strep&His Tag (MALS verified) | |
| Glycoprotein B/gB (EBV) | GLB-E52H3 | Epstein-Barr virus Envelope Glycoprotein B (gB), His Tag (MALS verified) | ||
| CMV | Glycoprotein B/gB (HCMV) | CMB-V5255 | HCMV Glycoprotein B (gB) Protein, Fc Tag | |
| Glycoprotein B/gB (HCMV) | CMB-V52H4 | HCMV (strain AD169) Glycoprotein B (gB), His Tag (MALS verified) | ||
| Glycoprotein B/gB (HCMV) | CMB-V52H5 | HCMV (strain Merlin) Glycoprotein B (gB), His Tag (MALS verified) | ||
| Glycoprotein H & Glycoprotein L/gH & gL (HCMV) | GHL-H5243 | HHV-5 (strain AD169) Glycoprotein H&L, His Tag (MALS verified) | ||
| Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) | GLO-H5283 | HHV-5 (strain AD169) gH&gL&gO, Twin-Strep Tag&Tag Free&His Tag | ||
| Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) | GLO-V5243 | Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (MALS verified) |
Herpesviruses Specific Antibodies
Advantages of products
High Sensitivity & Specificity: Antibodies exhibit both high sensitivity and specificity.
Recombinant Expression: Antibodies are produced via recombinant expression, offering good batch-to-batch stability.
Versatility: Suitable for detecting various herpesvirus antigen proteins or as an antibody standard.
Anti-Glycoprotein E VZV Antibody (SPR)
Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (1B10) (Cat. No. VZV-M544) captured on Protein A Chip can bind Varicella zoster virus (strain Oka vaccine) Envelope Glycoprotein E (gE), His Tag (Cat. No. GLE-V52H3) with an affinity constant of 5.63 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Anti-gC-HSV-2 (strain 333) Antibody (ELISA)
Immobilized HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (Cat. No. HSV-Y184) with a linear range of 0.098-6.25 ng/mL (QC tested).
| Product Type | Virus | Molecule | Cat. No. | Product Description |
|---|---|---|---|---|
| Antibody | VZV | Glycoprotein E/gE (VZV) | VZV-BLM545 | Biotinylated Monoclonal Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (4G2) |
| Glycoprotein E/gE (VZV) | VZV-M544 | Anti-Glycoprotein E (VZV) Antibody, Human IgG1 (1B10) (MALS verified) | ||
| HSV | Glycoprotein D/gD (HSV) | GLD-M609 | Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9) | |
| Glycoprotein D/gD (HSV) | GLD-Y111 | Monoclonal Glycoprotein D (HSV-2) Antibody, Mouse IgG1 (3H9) | ||
| Glycoprotein C/gC (HSV) | HSV-Y184 | Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) |
ELISA Kit for Antigen Quantification and Immunogenicity Evaluation
Advantages of products
High Sensitivity: The herpes virus antigen quantification assay kit has a maximum sensitivity of 78 pg/mL.
Wide Detection Range & Flexibility: Assay kits offer various detection ranges can meet the needs for various sample concentrations.
High Precision: The coefficient of variation (CV) for both intra-plate and inter-plate precision is ≤15%.
High Precision: The coefficient of variation (CV) for both intra-plate and inter-plate precision is ≤15%.
High Recovery Rate: The recovery rate for spiked samples is between 80% and 120%.
We offer kits for different species, including Human and Mouse, to meet the needs of immunological assessments in both preclinical and clinical stages.
Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit
Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay. Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using Biotin-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 78 pg/mL (QC tested).
Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.
ELISA- Anti-gC-HSV-2 (strain 333) Antibody Pair
The specific Anti-gC-HSV-2 (strain 333) Antibody pair (Clone:9A12/3A12) for the detection of HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3), linear range of 1.56-0.024ng/mL. (sandwich ELISA)
Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE)
For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.
| Product Type | Virus | Molecule | Cat. No. | Product Description |
|---|---|---|---|---|
| Antigen Quantification Assay Kit | VZV | Glycoprotein E/gE (VZV) | RAS-A102 | Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit |
| Glycoprotein E/gE (VZV) | RAS-A185 | Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit (For Vaccine Development) | ||
| Antibody Titer Assay Kit | VZV | Glycoprotein E/gE (VZV) | RAS-T103 | Mouse Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE) |
| Glycoprotein E/gE (VZV) | RAS-T132 | Human Anti-Varicella Zoster Virus Antibody IgG Titer Serologic Assay Kit (gE) |
Frequently Asked Questions
Among the numerous HSV antigens (such as gH & gL, gC, gD, gE, gB, etc.), which one is best target for vaccine development?
gB, as the most conserved fusion glycoprotein, plays a crucial role in driving the fusion of the viral envelope with the host cell membrane. gC and gB work synergistically to help concentrate viral particles on the surface of host cells. gD can specifically bind to host cells, while the gH/gL heterodimer can facilitate signaling, promoting the fusion of the viral envelope with the host cell membrane. In selecting candidate vaccine targets, one can choose based on their specific research focus and the characteristics of the antigen proteins.
How can the stability of test kit results be ensured?
All commercially available test kits undergo methodological validation based on the 9012 Guidelines for Quantitative Analysis of Biological Samples to ensure the stability of the test results.
For antigen quantitative detection test kits, can custom standards be used to replace the standard provided in the kit for quantification?
Replacement is possible, but you will need to conduct methodological validation and explore the quantitative range using your own standards.
What are the key differences between the VZV gE protein antigen quantitative detection kits RAS-A185 and RAS-A102?
RAS-A185 and RAS-A102 kits differ in sensitivity and colorimetric methods. The RAS-A185 uses HRP-Anti-Glycoprotein E (VZV) antibody for direct color development, while RAS-A102 employs Biotin-Anti-Glycoprotein E (VZV) antibody plus Streptavidin-HRP for color development. Both can be used for quantitative detection of VZV gE protein, but RAS-A102 has higher sensitivity, allowing you to choose the appropriate kit based on protein expression levels.
Resources
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[1] Lan, K., Luo, MH. Herpesviruses: epidemiology, pathogenesis, and interventions. Virol. Sin. 32, 347–348 (2017). https://doi.org/10.1007/s12250-017-4108-2
- Solutions for Herpesviruses Vaccines Development
- Multimeric Structural Antigen Designed based Native Conformation
- Herpesviruses Specific Antibodies
- ELISA Kit for Antigen Quantification and Immunogenicity Evaluation
- Frequently Asked Questions
- Resources