Factores de crecimiento para el cultivo de células madre pluripotentes inducidas
Culture and Expansion of iPSCs
Product List
GMP Human Laminin 521 Protein Hot
GMP Human Laminin 511 Protein Hot
Product Features
Performance - facilitate rapid expansion of hPSC and support efficient differentiation into a diverse array of specialized cell types.
Better Adhesion - maintain good adhesion characteristics at a concentration as low as 2μg/ml.
Stemness Maintenance - no spontaneous differentiation is observed after several passages of hPSC culture.
Lot-to-Lot Consistency - produced from a stable cell line, robust purification process, stringent QC.
Ready to Scale-up Supply - cGMP-compliant facility.
Supporting large-scale clinical supply - strictly adhere to the GMP management system, support multiple national regulations, and ensure stable supply for production.
Validation Data
Laminin 521 (Cat. No. GMP-LA5H24) effectively maintains the expansion of human iPSCs.
Laminin 521 (Cat. No. GMP-LA5H24) could maintain the stemness of iPSC after several passages
Normal karyotype (46, XX) was found in hiPSCs with Laminin 521(Cat. No. GMP-LA5H24) coating after 10 passages.
GMP Human Laminin 511 Protein (Cat. No. GMP-LA1H25) and Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283L) could maintain the stemness of iPSC at least Passage 5 and has similar performance. FACS data indicated that the iPSCs expressed high levels of pluripotency associated markers OCT4, SOX2, and SSEA4.
GMP Human Laminin 511 Protein (Cat. No. GMP-LA1H25) and Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283L) could maintain the stemness of iPSC at least Passage 5 and have similar performance. Immunofluorescence staining indicated that the iPSCs expressed high levels of pluripotency associated markers OCT4, SOX2, and NANOG.
FGF basic (Cat. No. GMP-FGCH17) could highly support stemness maintenance in ESC/iPSC compared to other companies.
GMP Human FGF basic Protein (Cat. No. GMP-FGCH17) and GMP Human TGF-Beta 1 Protein (Cat. No. GMP-TG1H25) could maintain the stemness of hiPSCs with high expression of stem cell genes OCT4, SOX2, and SSEA4 with GMP Human Laminin 521 Protein (Cat. No. GMP-LA5H24).
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Genome Editing of iPSCs
Product List
Product Features
High purity, high enzyme activity, high cleavage efficiency
Possesses nuclear localization signals to enhance editing efficiency
Aseptic, ultra-low endotoxin
Produced in GMP-compliant facilities and undergoes QC testing
Validation Data
Different amounts of Cas9 were incubated with the same amount of excess gRNA and plasmid for 60 minutes at 37°C. When using 400-200 ng Acro Cas9, the cutting efficiency is greater than 90%. In comparison, when using a 200 ng Competitor T, the cutting efficiency is only about 50%.
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Induced Differentiation of iPSCs
- iPSC-T Cell
- iPSC-NK Cell
- iPSC-Neuron Cell
- iPSC-Islet Cell
iPSC-T Cell
Product List GMP Available
Product Features
GMP Grade DLL4, VCAM1：Eliminate the need of feeder cells in culture systems and support differentiation to T cells
GMP Grade BMP-4: Significantly inducing iPSC derived HSPC differentiation during the process of iPSC differentiation into T/NK cells
Validated activity by iPSC to T cell differentiation
Production and quality control are carried out under strict GMP systems and comply with regulations from multiple countries
Comprehensive quality release verification, with 16 quality control indicators
Enhanced safety (sterile, no mycoplasma, no exogenous virus, animal free production system, and various impurities residual detection)
Pharmaceutical-grade production facility
Support for online and offline audits
Completion of FDA Drug Master File (DMF) registration
Validation Data
BMP4 (Cat. No. GMP-BM4H36), VEGF (Cat. No. GMP-VE5H23), SCF (Cat. No. GMP-SCFH25), TPO(Cat. No. GMP-THNH25) and FGF basic (Cat. No. GMP-FGCH17) could significantly induce iPSC derived HSPC differentiation, with high expression of CD34 and CD45 (HSPC markers) by flow cytometry analysis.
CD34+ CD45+ hematopoietic cells were seeded on GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) coated plates and differentiated for 14 days, then flow cytometry was used to detect the expression of T-cell progenitor markers, CD5 and CD7.
GMP Human DLL4 Protein, Fc Tag (Flagship) (Cat. No. GMP-DL4H27) and GMP Human VCAM-1 Protein, Fc Tag (Cat. No. GMP-VC1H25) together with other growth factors could induce the high percentage of CD7+ and CD5+ CD7+ T-cell progenitors formation.
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- Background
- Culture and Expansion of iPSCs
- Genome Editing of iPSCs
- Induced Differentiation of iPSCs
- Resources