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Product Details
Synonyms
Triggering receptor expressed on myeloid cells 2, TREM2, TREM-2
Source
Human TREM2, Fc Tag (TR2-H5254) is expressed from human 293 cells (HEK293). It contains AA His 19 - Ser 174 (Accession # Q9NZC2-1).
Predicted N-terminus: His 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human TREM2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TREM2, Fc Tag (Cat. No. TR2-H5254) is more than 85% and the molecular weight of this protein is around 90-125 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human TREM2, Fc Tag (Cat. No. TR2-H5254) at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody, Human IgG1 with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Human TREM2, Fc Tag (Cat. No. TR2-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-34, His Tag (Cat. No. IL4-H52H3) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
Immobilized Human TREM2, Fc Tag (Cat. No. TR2-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human APOE2 (R176C) Protein, His,Avitag (Cat. No. APE-H52E5) with a linear range of 0.005-2.5 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human TREM2, Fc Tag (Cat. No. TR2-H5254) on Protein A Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 66.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TREM2, Fc Tag (Cat. No. TR2-H5254) on Protein A Biosensor, can bind Human APOE2 (R154S, R176C) Protein, His Tag (Cat. No. APE-H5256) with an affinity constant of 17.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Triggering receptor expressed on myeloid cells 2
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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