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Product Details
Synonyms
Streptavidin, SA
Source
Streptavidin Protein-Alexa Fluor 647 (STN-NA119) is expressed from E. coli cells.
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa. The protein migrates as 12-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a tetramer.
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF647 to protein molar ratio is 3-6.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Streptavidin Protein-Alexa Fluor 647 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Streptavidin Protein-Alexa Fluor 647 (Cat. No. STN-NA119) was greater than 90% as determined by SEC-HPLC.
Bioactivity-FACS
5e5 of Jurkat cells were stained with 100 μL of 3 μg/mL of Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade and isotype control antibody respectively, washed and then followed with 100 μL of 1 μg/mL of Streptavidin Protein-Alexa Fluor 647 (Cat. No. STN-NA119) and analyzed with FACS. Alexa 647 signal was used to evaluate the binding activity (Routinely tested).
Protocol
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