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Product Details
Synonyms
SIGLEC10, MGC126774, PRO940, Siglec10, SLG2
Source
Human Siglec-10, Fc (L118A, G120A, E201A) Tag (SI0-H5255) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>85% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Siglec-10, Fc (L118A, G120A, E201A) Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.
Bioactivity-ELISA
Immobilized Human Siglec-10, Fc (L118A, G120A, E201A) Tag (Cat. No. SI0-H5255) at 5 μg/mL (100 μL/well) can bind Anti-Siglec-10 Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human CD32a (R167), His Tag (Cat. No. CDA-H5221) on HIS1K Biosensor, can bind Human Siglec-10, Fc (L118A, G120A, E201A) Tag (Cat. No. SI0-H5255) with an affinity constant of 37.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human CD64, His Tag (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Human Siglec-10, Fc (L118A, G120A, E201A) Tag (Cat. No. SI0-H5255) with an affinity constant of 42.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Sialic acid-binding Ig-like lectin 10
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 3 Clinical
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