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Product Details
Synonyms
IL-23 alpha & IL-12 beta
Source
Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (ILB-M82W3) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 & Met 23 - Ser 335 (Accession # Q9EQ14 & P43432).
Predicted N-terminus: Val 22 & Met 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 23.2 kDa (IL23A) and 35.8 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL12B contains no tag at the C-terminus. The protein migrates as 27 kDa, 42 kDa and 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-M82W3) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-M82W3) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Immobilized Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-M82W3) with a linear range of 0.002-0.313 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) captured on Protein A Chip can bind Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-M82W3) with an affinity constant of 1.84 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) captured on Protein A Chip can bind Biotinylated Mouse IL23A & IL12B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ILB-M82W3) with an affinity constant of 11.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-12 subunit beta
- Category：
- Approved Drugs：
7 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Approved
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