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Product Details
Synonyms
FCGR2B, C, CD32b, c, FcRII-b, c, Fc-gamma RII-b, c, Fc-gamma-RIIb, c, CD32, FCG2, IGFR2, CDw32
Source
Human CD32b/c Protein, Strep Tag (CDB-H5284) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1).
Predicted N-terminus: Ala 46
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a twin strep tag at the C-terminus.
The protein has a calculated MW of 22.7 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD32b/c Protein, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) is more than 95% and the molecular weight of this protein is around 26-38 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) immobilized on CM5 Chip can bind Rituximab with an affinity constant of 10.2 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Rituximab immobilized on CM5 Chip can bind Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) with an affinity constant of 5.18 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
Recent Advances
Drug Development Progress- English Name：
Low affinity immunoglobulin gamma Fc region receptor II-b
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Phase 3 Clinical
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