Mouse CD27 Ligand / CD70 Protein, His Tag, active trimer (MALS verified)

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Cat. No. / Size
Price
Qty
CDL-M5245-100ug
$360.00
CDL-M5245-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

  • Source

    Mouse CD27 Ligand Protein, His Tag (CDL-M5245) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    CD27 Ligand Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 27-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD27 Ligand SDS-PAGE

    Mouse CD27 Ligand Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD27 Ligand SEC-MALS

    The purity of Mouse CD27 Ligand Protein, His Tag (Cat. No. CDL-M5245) is more than 95% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD27 Ligand ELISA

    Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, His Tag (Cat. No. CDL-M5245) with a linear range of 0.1-4 ng/mL (QC tested).

    Protocol

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Background

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

Recent Advances

 
Drug Development Progress
  • English Name:

    CD70 antigen

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    37 Details

  • Highest Development Stage:

    Phase 2 Clinical

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