Mouse Beta-NGF Protein, Tag Free, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
BEF-M5213-20ug
$90.00
BEF-M5213-50ug
$135.00
BEF-M5213-500ug
$805.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    NGF, NGFB, Beta-NGF, HSAN5, MGC161426, MGC161428

  • Source

    Mouse Beta-NGF Protein, Tag Free (BEF-M5213) is expressed from human 293 cells (HEK293). It contains AA Ser 122 - Gly 241 (Accession # P01139).

    Predicted N-terminus: Ser 122

    Request for sequence
  • Molecular Characterization

    Beta-NGF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 13.5 kDa. The protein migrates as <14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Beta-NGF SDS-PAGE

    Mouse Beta-NGF Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Beta-NGF SEC-MALS

    The purity of Mouse Beta-NGF Protein, Tag Free (Cat. No. BEF-M5213) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Beta-NGF ELISA

    Immobilized Mouse Beta-NGF Protein, Tag Free (Cat. No. BEF-M5213) at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Fc Tag (Cat. No. TRA-H5254) with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol

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Background

Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways.

Recent Advances

 
Drug Development Progress
  • English Name:

    Nerve growth factor

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    14 Details

  • Highest Development Stage:

    NDA/BLA

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