Monoclonal Anti-Human Phospho-Tau (Thr181) Antibody, Mouse IgG2a (8D8C10)

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Cat. No. / Size
Price
Qty
PT1-Y2073-100ug
$355.00
PT1-Y2073-1mg (500ug X 2)
$2040.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Human Phospho-Tau (Thr181) Antibody, Mouse IgG2a (8D8C10) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    8D8C10

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Immunogen

    Recombinant polypeptide protein

  • Specificity

    Specifically recognizes Human p-tau181 Protein.

  • Application

    Application
    Recommended Usage
    IF
    1:50-1:500
  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Immunofluorescence

     Tau IMMUNOFLUORESCENCE

    2D cell staining: Immunofluorescent staining (10X) of phosphorylated tau in treated SH-SY5Y neuroblastoma cells with purified PT1-Y2073 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.

    Protocol
  • Western Blot

     Tau WESTERN BLOT
  • Detection of Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a(8D8C10), Mouse IgG2a | Kappa,HEK by Western Blot. Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a(8D8C10), Mouse IgG2a | Kappa,HEK at 10.0 ug/ml dilution + Phosphorylated SH-SY5Y cell lysate, Mouse Tag at 20uL.
    Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecific(1:1500).
    Predicted band size: 65 kDa 12% Bis-Tris Protein Gel.
    The recommended dilution ratio is 10.0 ug/ml.

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Background

Tau, the microtubule‐associated protein, forms insoluble filaments that accumulate as neurofibrillary tangles in Alzheimer's disease (AD) and related tauopathies. Under physiological conditions, tau regulates the assembly and maintenance of the structural stability of microtubules. In the diseased brain, however, tau becomes abnormally hyperphosphorylated, which ultimately causes the microtubules to disassemble, and the free tau molecules aggregate into paired helical filaments.

Recent Advances

 
Drug Development Progress
  • English Name:

    Microtubule-associated protein tau

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    46 Details

  • Highest Development Stage:

    Approved

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