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Product Details
Synonyms
TSLP
Source
Human TSLP Protein, Fc Tag (TSP-H5253) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).
Predicted N-terminus: Tyr 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.4 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human TSLP Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TSLP Protein, Fc Tag (Cat. No. TSP-H5253) is more than 90% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TSLP Protein, Fc Tag (Cat. No. TSP-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
Immobilized Human TSLP Protein, Fc Tag (Cat. No. TSP-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.06-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human TSLP R, Fc Tag (Cat. No. TSR-H525a) immobilized on CM5 Chip can bind Human TSLP Protein, Fc Tag (Cat. No. TSP-H5253) with an affinity constant of 47.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Thymic stromal lymphopoietin
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Approved
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