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Product Details
Synonyms
TSLP
Source
Cynomolgus TSLP (R127A, R130S) Protein, His Tag (TSP-C52H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A7N9CAT7-1 (R127A, R130S)).
Predicted N-terminus: Tyr 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 21-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus TSLP (R127A, R130S) Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Cynomolgus TSLP (R127A, R130S) Protein, His Tag (Cat. No. TSP-C52H4) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Cynomolgus TSLP (R127A, R130S), His Tag (Cat. No. TSP-C52H4) with a linear range of 1-4 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus TSLP (R127A, R130S), His Tag (Cat. No. TSP-C52H4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Thymic stromal lymphopoietin
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Approved
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