Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Mouse TL1A , His Tag (TLA-M5243) is expressed from human 293 cells (HEK293). It contains AA Ala 61 - Leu 252 (Accession # Q5UBV8-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 23.4 kDa. The protein migrates as 27-29 kDa and 30-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Mouse TL1A , His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC

The purity of Mouse TL1A , His Tag (Cat. No. TLA-M5243) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA

Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Mouse TL1A , His Tag (Cat. No. TLA-M5243) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Immobilized Mouse TL1A , His Tag (Cat. No. TLA-M5243) at 5 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 0.5-8 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Mouse DR3 Protein, His Tag (Cat. No. DR3-M52H3) immobilized on CM5 Chip can bind Mouse TL1A , His Tag (Cat. No. TLA-M5243) with an affinity constant of 17.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
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