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Product Details
Synonyms
SIGLEC6, CD327, CD33L, CD33L1, OBBP1, OB-BP1, CDw327
Source
Human Siglec-6, Fc Tag (SI6-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Val 320 (Accession # AAH35359).
Predicted N-terminus: Gln 16
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 60.3 kDa. The protein migrates as 80-100 kDa under reducing (R) condition, and 130-150 kDa and 250-300 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Siglec-6, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Siglec-6 Antibody, Rabbit IgG with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Human siglec-6/D327 Antibody, Sheep IgG with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
C***u2022-06-25
LikesThe item was expedited very quickly which worked out with our timeline. We are in the process of using the Siglec-6 protein in one of our in-vivo study for immunization. The material itself did not dissolve in complete freund's adjuvant but it was easily made into a fine suspension with the help of some PBS. Overall, I would say the material is easy to work with and the Acro is quick at responding if we have any issues.
Background
Recent Advances
Drug Development Progress- English Name：
Sialic acid-binding Ig-like lectin 6
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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