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Product Details
Synonyms
CD22, SIGLEC2, BL-CAM, SIGLEC-2, Siglec2, SIGLEC2FLJ22814
Source
FITC-Labeled Human Siglec-2, Fc Tag (CD2-HF254) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2, Fc Tag (Cat. No. CD2-H5253).
Predicted N-terminus: Asp 20
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 101.6 kDa. The protein migrates as 130-150 kDa and >250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 3.5-5.5.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
FITC-Labeled Human Siglec-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Monoclonal Anti-Human Siglec-2 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2, Fc Tag (Cat. No. CD2-HF254) with a linear range of 0.1-2.5 μg/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD22 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human Siglec-2, Fc Tag (Cat. No.CD2-HF254) and negative control protein respectively, FITC signal was used to evaluate the binding activity (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CD22
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
73 Details
- Highest Development Stage：
Approved
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