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Human Siglec-10 Protein, Fc Tag, low endotoxin

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Cat. No. / Size
Price
Qty
SI0-H5253-100ug
$395.00
SI0-H5253-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    SIGLEC10, MGC126774, PRO940, Siglec10, SLG2

  • Source

    Human Siglec-10, Fc Tag (SI0-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

    Predicted N-terminus: Met 17

    Request for sequence
  • Molecular Characterization

    Siglec-10 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 84.6 kDa. The protein migrates as 90-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Siglec-10 SDS-PAGE

    Human Siglec-10, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Siglec-10 ELISA

    Immobilized Human Siglec-10, Fc Tag (Cat. No. SI0-H5253) at 5 μg/mL (100 μL/well) can bind Anti-Human Siglec-10 MAb, human IgG with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

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Background

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

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Drug Development Progress
  • English Name:

    Sialic acid-binding Ig-like lectin 10

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    3 Details

  • Highest Development Stage:

    Phase 3 Clinical

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  • Background