Save Product
Product Details
Synonyms
FOLH1, PSMA, GIG27, FOLH, NAALAD1, PSM, NAALADase I, GCPII, FGCP
Source
FITC-Labeled Human PSMA, His Tag (PSA-HF244) is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750 (Accession # Q04609-1 ). It is the FITC labeled form of Human PSMA, His Tag (Cat. No. PSA-H52H3).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 81.4 kDa. The protein migrates as 85-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 1-3.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 500 mM NaCl, pH6.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
FITC-Labeled Human PSMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Monoclonal Anti-Human PSMA Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human PSMA, His Tag (Cat. No. PSA-HF244) with a linear range of 0.005-0.16 μg/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-PSMA CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human PSMA Protein, His Tag (Cat. No. PSA-HF244) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Prostate-Specific Membrane Antigen
- Category：
- Approved Drugs：
6 Details
- Drugs in Clinical Trials：
159 Details
- Highest Development Stage：
Approved
Contact Us
Drug Candidate Licensing
