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Human Perlecan Protein, His Tag, low endotoxin

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Cat. No. / Size
Price
Qty
PEN-H5245-100ug
$360.00
PEN-H5245-1mg
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    Perlecan

  • Source

    Human Perlecan Protein, His Tag (PEN-H5245) is expressed from human 293 cells (HEK293). It contains AA Val 1857 - Pro 1956 (Accession # P98160).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    Perlecan Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 12.2 kDa. The protein migrates as 11-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Perlecan SDS-PAGE

    Human Perlecan Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

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Background

Siglec-15 is a transmembrane glycoprotein belonging to the Siglec family, recognized for its sialic acid-binding properties and role in immune regulation. Structurally, its extracellular domain contains two Ig-like domains, and it functionally associates with the signaling adaptors DAP12 and DAP10. Siglec-15 is expressed on immune cells, including osteoclasts, macrophages, and dendritic cells. It exerts its function by binding to its ligand, the sialyl-Tn antigen. This interaction is critical for inducing TGF-beta production by tumor-associated macrophages and plays an essential role in osteoclast formation and TRANCE/RANK Ligand signaling.

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