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Product Details
Synonyms
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
Source
Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
Predicted N-terminus: Gln 31 & Ser 153
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 24 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human PCSK9, His Tag (Cat. No. PC9-H5223) is more than 95% and the molecular weight of this protein is around 70-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 5 μg/mL (100 μL/well) can bind Human LDL R, Fc Tag with a linear range of 10-156 ng/mL (QC tested).
Protocol
Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 2 μg/mL (100 μL/well) on an Nickel Coated plate can bind Human PCSK9 Mab (RN316), Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 1 μg/mL (100 μL/well) on Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) precoated (0.1 μg/well) plate can bind Evolocumab with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Evolocumab (Cat. No. HY-P9930) captured on Protein A Chip can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 1.73 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 2.17 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded PCSK9 Mab, Human IgG1 on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 88.6 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Preguntas Frecuentes
- Product
What key experimental conditions should be considered for PCSK9–LDLR binding assays?
It is recommended to perform PCSK9–LDLR binding assays under acidic conditions, as the interaction exhibits higher activity in acidic environments compared to neutral ones. Reference: Effects of pH and Low Density Lipoprotein (LDL) on PCSK9-dependent LDL Receptor Regulation.
Background
Recent Advances
Drug Development Progress- English Name：
Proprotein convertase subtilisin kexin type 9
- Category：
- Approved Drugs：
8 Details
- Drugs in Clinical Trials：
35 Details
- Highest Development Stage：
Approved
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