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Product Details
Synonyms
TNFRSF4, OX40, CD134, OX40L receptor, ACT35, TXGP1L
Source
Human OX40 Protein, Mouse IgG2a Fc Tag (OX0-H5252) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # P43489-1).
Predicted N-terminus: Leu 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 47.1 kDa. The protein migrates as 58-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human OX40 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human OX40 Protein, Mouse IgG2a Fc Tag (Cat. No. OX0-H5252) is more than 85% and the molecular weight of this protein is around 100-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human OX40 Ligand, His Tag (Cat. No. OXL-H52Q8) at 2 μg/mL (100 μL/well) can bind Human OX40 Protein, Mouse IgG2a Fc Tag (Cat. No. OX0-H5252) with a linear range of 0.78-25 ng/mL (QC tested).
Protocol
Bioactivity-FACS
FACS assay shows that recombinant Human OX40 Protein, Mouse IgG2a Fc Tag (Cat. No. OX0-H5252) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.01 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 4
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Phase 3 Clinical
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