Human NT-4 / Neurotrophin-4 Protein, Tag Free, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
NT4-H5114-20ug
$260.00
NT4-H5114-50ug
$385.00
NT4-H5114-500ug (250ug X 2)
$2320.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    neurotrophic factor 4, neurotrophic factor 5, neurotrophin 4, neurotrophin 5, neurotrophin-5, NT4, NT-4, 5, NT-5, NTF4, NTF5, GLC10

  • Source

    Human NT-4, Tag Free (NT4-H5114) is expressed from E. coli cells. It contains AA Gly 81 - Ala 210 (Accession # P34130-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    NT-4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 14.1 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    NT-4 SDS-PAGE

    Human NT-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    NT-4 SEC-MALS

    The purity of Human NT-4, Tag Free (Cat. No. NT4-H5114) is more than 95% and the molecular weight of this protein is around 28-40 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     NT-4 ELISA

    Immobilized Human NT-4, Tag Free (Cat. No. NT4-H5114) at 5 μg/mL (100 μL/well) can bind Human TrkB, Fc Tag (Cat. No. NT2-H5254) with a linear range of 2-10 ng/mL (QC tested).

    Protocol

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Background

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. Their functions including supporting the survival and differentiation of existing neurons, encouraging the growth and differentiation of new neurons and synapses, are required in both the central and the peripheral nervous systems.

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  • Background