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Product Details
Synonyms
NCR1, LY94, CD335, NK-p46, hNKp46
Source
Human NKp46, His Tag (NC1-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # AAH64806.1).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.3 kDa. The protein migrates as 36-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human NKp46, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human NKp46, His Tag (Cat. No. NC1-H52H4) is more than 95% and the molecular weight of this protein is around 32-48 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human NKp46, His Tag (Cat. No. NC1-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Natural cytotoxicity triggering receptor 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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