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Product Details
Synonyms
LDLR, FH, FHC, LDLCQ2
Source
Human LDL R, Fc Tag (LDR-H5251) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Arg 788 (Accession # P01130-1).
Predicted N-terminus: Ala 22
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Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 111.2 kDa. The protein migrates as 120-125 kDa and 140-150 kDa under reducing (R) condition, and 270-330 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human LDL R, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human LDL R, Fc Tag (Cat. No. LDR-H5251) is more than 90% and the molecular weight of this protein is around 235-265 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human PCSK9 (D374Y), His Tag (Cat. No. PCY-H5225) at 5 μg/mL (100 μL/well) can bind Human LDL R, Fc Tag (Cat. No. LDR-H5251) with a linear range of 0.005-1.25 μg/mL (QC tested).
Protocol
Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5251) at 5 μg/mL (100 μL/well) can bind Human PCSK9 (D374Y), His Tag (Cat. No. PCY-H5225) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
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Preguntas Frecuentes
- Product
What key experimental conditions should be considered for PCSK9–LDLR binding assays?
It is recommended to perform PCSK9–LDLR binding assays under acidic conditions, as the interaction exhibits higher activity in acidic environments compared to neutral ones. Reference: Effects of pH and Low Density Lipoprotein (LDL) on PCSK9-dependent LDL Receptor Regulation.
Background
Recent Advances
Drug Development Progress- English Name：
LDL receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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