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Product Details
Synonyms
Integrin alpha 1 beta 1, ITGA1&ITGB1
Source
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wb) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Pro 1141 (ITGA1) & Gln 21 - Asp 728 (ITGB1) (Accession # P56199-1 (ITGA1) & P05556-1 (ITGB1)).
Predicted N-terminus: Phe 29 (ITGA1) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 170-200 kDa (ITGA1) and 90-105 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 0.2 M Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) is more than 90% and the molecular weight of this protein is around 230-280 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wb) with a linear range of 1-20 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha1/beta1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Pending
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