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Cynomolgus IL-6 Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
IL6-C5249-50ug
$360.00
IL6-C5249-1mg (250ug X 4)
$2945.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    IL6, Interleukin-6, BSF2, HSF, IFNB2

  • Source

    Cynomolgus IL-6, His Tag (IL6-C5249) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Met 212 (Accession # P79341-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    IL-6 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 22.9 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-6 SDS-PAGE

    Cynomolgus IL-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-6 SEC-MALS

    The purity of Cynomolgus IL-6, His Tag (Cat. No. IL6-C5249) is more than 95% and the molecular weight of this protein is around 20-35 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-6 ELISA

    Immobilized Cynomolgus IL-6, His Tag (Cat. No. IL6-C5249) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Fc,Avitag (Cat. No. ILR-H82F9) with a linear range of 0.6-39 ng/mL (QC tested).

    Protocol

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Background

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-6

  • Category:

  • Approved Drugs:

    5 Details

  • Drugs in Clinical Trials:

    22 Details

  • Highest Development Stage:

    Approved

Drug Candidate Licensing
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  • Background