Save Product
Product Details
Synonyms
IL18BP, IL18BPa, Tadekinig-alfa
Source
Human IL-18BP, His Tag (ILP-H5222) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
Predicted N-terminus: Thr 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.5 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human IL-18BP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-18BP, His Tag (Cat. No. ILP-H5222) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) immobilized on CM5 Chip can bind Human IL-18BP, His Tag (Cat. No. ILP-H5222) with an affinity constant of 0.319 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-CELL BASE
Human IL-18BP, His Tag (Cat. No. ILP-H5222) inhibits the IL-18-induced IFN-gamma secretion by KG‑1 cells. The ED50 for this effect is 0.019-0.053 µg/mL in the presence of 30 ng/mL of Recombinant Human IL‑18 (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Contact Us
Drug Candidate Licensing
