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Product Details
Synonyms
IFN-alpha 2b, IFNA2B, IFN-alpha 2 (alpha 2b), interferon alpha 2b
Source
Human IFN-alpha 2b, Fc Tag (IFB-H5253) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 188 (Accession # P01563-1).
Predicted N-terminus: Cys 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.7 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IFN-alpha 2b, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IFN-alpha 2b, Fc Tag (Cat. No. IFB-H5253) is more than 95% and the molecular weight of this protein is around 95-105 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-alpha 2b, Fc Tag (Cat. No. IFB-H5253) with a linear range of 2-10 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human IFN-alpha 2b, Fc Tag (Cat. No. IFB-H5253) immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IFNAR1, His Tag (Cat. No. IF1-H5225) on NTA Biosensor, can bind Human IFN-alpha 2b Protein, Fc Tag (Cat. No. IFB-H5253) with an affinity constant of 0.689 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Mouse IFN-alpha / beta R2, His Tag (Cat. No. IF2-M5225) on NTA Biosensor, can bind Human IFN-alpha 2b Protein, Fc Tag (Cat. No. IFB-H5253) with an affinity constant of 1.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interferon alpha-2
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
9 Details
- Highest Development Stage：
Approved
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