Product Details
Synonyms
HLA-A*1101 & B2M & KRAS (VVVGAGGVGK)
Source
PE-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Tetramer Protein (HLS-HP2H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGAGGVGK).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequenceMolecular Characterization
PE-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Tetramer Protein is assembled by biotinylated monomer (HLS-H82E6) and PE-labeled streptavidin.
Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide. Biotinylated Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA

Immobilized PE-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Tetramer Protein (Cat. No. HLS-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized PE-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Tetramer Protein (Cat. No. HLS-HP2H6) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
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