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Product Details
Synonyms
MET, AUTS9, HGFR, RCCP2, c-Met
Source
Human HGF R, Fc Tag (MET-H5256) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).
Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain Fc chimera)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus.
The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa (α chain) and 95.3 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 106-116 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human HGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human HGF R, Fc Tag (Cat. No. MET-H5256) is more than 95% and the molecular weight of this protein is around 280-340 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human HGF, His Tag (Cat. No. HGF-H52H3) at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
Immobilized Human HGF R, Fc Tag (Cat. No. MET-H5256) at 5 μg/mL (100 μL/well) can bind Human HGF, His Tag (Cat. No. HGF-H52H3) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Hepatocyte growth factor receptor
- Category：
- Approved Drugs：
13 Details
- Drugs in Clinical Trials：
74 Details
- Highest Development Stage：
Approved
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