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Product Details
Synonyms
ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1
Source
PE-Labeled Human Her2, His Tag (HE2-HP2E3) is produced via conjugation of PE to Human Her2, His Tag under optimal conditions. Human Her2, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
Predicted N-terminus: Thr 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 73.0 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Evaluation of anti-HER2 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS
5e5 of anti-Her2 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Her2, His Tag (Cat. No. HE2-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Customer Reviews Writing Reviews
I***l2024-09-27
LikesWe used the PE-labeled recombinant ErbB2 protein to detect our HER2-specific CAR constructs. The protein performed exceptionally well, providing a strong and consistent fluorescence signal when analyzing stained samples via flow cytometry. The staining was highly specific, since we detected no background-signal on parental cell-lines lacking the CAR. This is in contrast to staining of CAR expressing cells using ErbB2-Fc in combination with secondary antibodies which often results in higher non-specific signals and is more time consuming. Hence, ErbB2-PE is a valuable tool to detect ErbB2-specific CAR expression via Flow cytometry.
Background
Recent Advances
Drug Development Progress- English Name：
Receptor protein-tyrosine kinase erbB-2
- Category：
- Approved Drugs：
47 Details
- Drugs in Clinical Trials：
238 Details
- Highest Development Stage：
Approved
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