Product Details
Synonyms
HA, Hemagglutinin
Source
Influenza B virus (Pennsylvania/14/2025) HA (B/Victoria lineage) Protein, His Tag (HAB-V52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Thr 545 (Accession # EPI_ISL_20077099, GISAID).
Predicted N-terminus: Asp 16
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.8 kDa . The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 0.1 M Sodium citrate, 0.5 M Arginine, Glycerol, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Influenza B virus (Pennsylvania/14/2025) HA (B/Victoria lineage) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Influenza B virus (Pennsylvania/14/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H3) is more than 90% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza B virus (Pennsylvania/14/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Influenza B Hemagglutinin / HA Antibody, Rabbit Mab (QC tested).
Protocol
Bioactivity-SPR

Influenza B Hemagglutinin / HA Antibody, Rabbit Mab immobilized on CM5 Chip can bind Influenza B virus (Pennsylvania/14/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H3) with an affinity constant of 0.574 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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