Cynomolgus GPRC5D Protein, Flag,His Tag (Nanodisc)

Application

1. Cell based assay (FACS or functional assay)
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

GPRC5D, G-protein coupled receptor family C group 5 member D

Source

Nanodisc Cynomolgus GPRC5D Protein, Flag,His Tag (GPD-C82D4) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # A0A2K5W6I7-1).

Predicted N-terminus: Asp

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus. The protein has a calculated MW of 51.9 kDa. The protein migrates as 25 kDa and 37 kDa under reducing (R) condition (SDS-PAGE).

*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.

1. Structure

   

   Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

2. Synthesis Process

   

   Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

Purity

>80% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process