LCMV Glycoprotein C (R262A) Protein, His Tag

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Cat. No. / Size
Price
Qty
CLN-L52H2-100ug
$390.00
CLN-L52H2-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glycoprotein C/GP-C (LCMV)

  • Source

    LCMV Glycoprotein C (R262A) Protein, His Tag (CLN-L52H2) is expressed from human 293 cells (HEK293). It contains AA Met 59 - Asp 438 (Accession # AJ297484.1 (R262A)).

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  • Molecular Characterization

    Glycoprotein C/GP-C (LCMV) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 66-70 kDa and 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glycoprotein C/GP-C (LCMV) SDS-PAGE

    LCMV Glycoprotein C (R262A) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

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Background

Lymphocytic choriomeningitis virus (LCMV) is a spherical enveloped virus with an RNA genome consisting of two negative single-stranded RNA segments, the large (L) strand and the small (S) strand. The L strand encodes the virus polymerase (L) and a small zinc finger motif protein (Z); the smaller (S) segment encodes the two main structural proteins: virus nucleoprotein (NP) and glycoprotein (GP) precursor (GPC). The GP of LCMV serves as virus attachment protein to its receptor on host cells and is a key determinant for cell tropism, pathogenesis, and epidemiology of the virus. The GP undergoes post-translational cleavage and results in the synthesis of two mature virion glycoproteins, GP-1 (40 to 46 kDa) and GP-2 (35 kDa). The spikes present on the virion envelope are dictated by tetramer formation of GP-1 and GP-2. The peripheral GP-1 is implicated in receptor binding, and the transmembrane GP-2 is structurally similar to the fusion active membrane proximal portions of the glycoproteins of other enveloped viruses.

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