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Product Details
Synonyms
AITR, GITR, TNFRSF18, CD357
Source
Human GITR, His Tag (GIR-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
Predicted N-terminus: Gln 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.4 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human GITR, His Tag (Cat. No. GIR-H5228) is more than 95% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human GITR Ligand, Avitag,Fc Tag (Cat. No. GIL-H82F8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human GITR, His Tag (Cat. No. GIR-H5228) with a linear range of 0.6-39 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
1***32019-09-27
LikesI have been using Acro's products for a long time. Whenever there is some concerns on product or technical support is needed, it always gets solved in time. I used the GITR protein for SPR experiments. I have done 6 concentration gradients, and the fitted curve was still very good. The data also met my expectations. I hope we will continue having a good cooperation with ACRO moving forward.
Background
Recent Advances
Drug Development Progress- English Name：
Glucocorticoid-induced tumor necrosis factor receptor-related protein
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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