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Product Details
Synonyms
FGF R3 (IIIc), FGF R3C, CD333
Source
Human FGF R3 (IIIc), Fc Tag (FGC-H5256) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 375 (Accession # P22607-1).
Predicted N-terminus: Glu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.6 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human FGF R3 (IIIc), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) is more than 95% and the molecular weight of this protein is around 148-180 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human FGF R3 (IIIc), Fc Tag immobilized (Cat. No. FGC-H5256) on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 0.44 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) immobilized on CM5 Chip can bind Human FGF-9, Fc Tag (Cat. No. FG9-H4267) with an affinity constant of 10.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) on Protein A Biosensor, can bind Human FGF-9, His Tag (Cat. No. FG9-H52H3) with an affinity constant of 19.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Fibroblast growth factor receptor 3
- Category：
- Approved Drugs：
9 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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