Product Details
Synonyms
TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1
Source
Mouse DR3 Protein, His Tag (DR3-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Met 196 (Accession # B1AWN9-1).
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.7 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE

Mouse DR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC

The purity of Mouse DR3 Protein, His Tag (Cat. No. DR3-M52H3) was greater than 95% as determined by SEC-HPLC.
Bioactivity-SPR

Mouse DR3 Protein, His Tag (Cat. No. DR3-M52H3) immobilized on CM5 Chip can bind Mouse TL1A , His Tag (Cat. No. TLA-M5243) with an affinity constant of 17.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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