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Product Details
Synonyms
CD8A, CD8, Leu2, MAL, p32
Source
Cynomolgus CD8 alpha Protein, His Tag (CDA-C52H4) is expressed from human 293 cells (HEK293). It contains AA Asn 63 - Asp 223 (Accession # XP_005575419.2).
Predicted N-terminus: Asn 63
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.6 kDa. The protein migrates as 20-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus CD8 alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD8 alpha Protein, His Tag (Cat. No. CDA-C52H4) is more than 95% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Cynomolgus CD8 alpha Protein, His Tag (Cat. No. CDA-C52H4) at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Cluster of differentiation 8
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
18 Details
- Highest Development Stage：
Phase 3 Clinical
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