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Product Details
Synonyms
CD47, MER6, IAP, OA3
Source
Mouse CD47, His Tag (CD7-M522b) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Lys 140 (Accession # Q61735-1).
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.7 kDa. The protein migrates as 28-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse CD47, His Tag (Cat. No. CD7-M522b) is more than 95% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse SIRP alpha Protein, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 10 μg/mL (100 μL/well) can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with a linear range of 0.078-5 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) immobilized on CM5 Chip can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with an affinity constant of 2.52 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
L***z2026-02-12
LikesI have been using the protein for ELISA assays, andit has met our experimental requirements without any issues. Low doses are sufficient for coating, which makes the assays cost-effective while still providing strong and consistent signal. The shipping was fast and the product arrived in excellent condition.
Background
Recent Advances
Drug Development Progress- English Name：
Cluster of differentiation 47
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
53 Details
- Highest Development Stage：
Phase 3 Clinical
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