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Product Details
Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-C52W4) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5 (CD3E) & Q95LI8 (CD3D)).
Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) and Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 15 kDa, 17 kDa, 27-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) is more than 95% and the molecular weight of this protein is around 33-50 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) at 2 μg/mL, add Human anti-BCMA×CD3 scFv, C-Fc and then add Biotinylated Human BCMA, Fc,Avitag, premium grade (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 2.49 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 64.4 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD3×BCMA scFV on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-C52W4) with an affinity constant of 8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***12022-08-09
LikesThe purchased protein has been verified to have good biological activity by ELISA assay, and the species can be used for subsequent screening and functional assays of hybridoma monoclonal antibodies.
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