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Product Details
Synonyms
CD30-L, CD153, TNFSF8, CD30L, CD30LG, CD153 antigen, CD30 antigen ligand, CD30 Ligand
Source
Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (CDL-H525b) is expressed from human 293 cells (HEK293). It contains AA Gln 63 - Asp 234 (Accession # P32971-1).
Predicted N-terminus: Glu
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 46.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD30 Ligand Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human CD30, His Tag (Cat. No. CD0-H5229) at 2 μg/mL (100 μL/well) can bind Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-H525b) with a linear range of 0.4-25 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD30 Protein, Avitag,His Tag (Cat. No. CD0-H82E6) on SA Biosensor, can bind Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-H525b) with an affinity constant of 114 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS analysis shows that Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-H525b) can bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 0.1 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human CD30 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-H525b) to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralizing anti-CD30 antibody. The concentration of Human CD30 Ligand used is 0.1 μg/mL. The IC50 is 0.2064 μg/mL (Routinely tested).
Protocol
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Background
(TNFR) superfamilies. In humans, CD30L is expressed at high levels on activated T cells. There is conflicting evidence with regard to cell surface expression of CD30L on primary human B cells. An initial report of constitutive CD30L expression on peripheral blood B cells33 has not been substantiated.
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 8
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- Highest Development Stage：
Preclinical
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