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Product Details
Synonyms
TNFRSF8, CD30, D1S166E, Ki-1
Source
FITC-Labeled Human CD30, His Tag (CD0-HF2H4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2). It is the FITC labeled form of Human CD30, His Tag (Cat. No. CD0-H52H5).
Predicted N-terminus: Phe 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 40.4 kDa. The protein migrates as 60-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
Protein Ratio
The FITC to protein molar ratio is 2.5-4.5.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
FITC-Labeled Human CD30, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Monoclonal Anti-Human CD30 Human Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD30, His Tag (Cat. No. CD0-HF2H4) with a linear range of 10-313 ng/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of anti-CD30 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Human CD30, His Tag (Cat. No.CD0-HF2H4) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).
Protocol
Customer Reviews Writing Reviews
R*** 2021-09-22
LikesOur research group work with CAR-T therapy for the treatment of hematological malignancies, and use the Fitc-Labeled Human CD30 protein for detection of CAR30 cells in patients by flow cytometry. Our results are fantastic, because the observe consistency and a reproducible experiments by flow cytometry. The logistic speed is very effective and allows us to work always up to date with our projects.
Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 8
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
22 Details
- Highest Development Stage：
Approved
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