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Product Details
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. The protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus CD3 epsilon, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) is more than 95% and the molecular weight of this protein is around 32-50 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilize Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA), and then add Biotinylated Human BCMA, Fc,Avitag (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection is performed using HRP-conjugated streptavidin. Binding occurs at a linear range of 1-5 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Bispecific T-cell Engager captured on Protein A Chip can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.674 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-Human CD3 mAb, mouse IgG1 (Clone # SP3541-2) on AMC Biosensor, can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.305 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Bispecific T-cell Engager (CD3 X BCMA) on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H525541), can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.113 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD3 epsilon chain
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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