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Mouse CD27 Ligand / CD70 Protein, Mouse IgG2a Fc Tag, low endotoxin

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Cat. No. / Size
Price
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CDL-M5251-100ug
$310.00
CDL-M5251-1mg
$2245.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

  • Source

    Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (CDL-M5251) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).

    Predicted N-terminus: Glu

    Request for sequence
  • Molecular Characterization

    CD27 Ligand Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the N-terminus.

    The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, 25 mM Arginine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD27 Ligand SDS-PAGE

    Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     CD27 Ligand ELISA

    Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 1 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-M5251) with a linear range of 0.3-5 ng/mL (QC tested).

    Protocol

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Background

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

Recent Advances

 
Drug Development Progress
  • English Name:

    CD70 antigen

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    37 Details

  • Highest Development Stage:

    Phase 2 Clinical

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